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Novel application of stem cell-derived factors for periodontal regeneration.Biochem Biophys Res Commun. 2012 Nov 30.
Novel application of stem cell-derived factors for periodontal regeneration.
Inukai T, Katagiri W, Yoshimi R, Osugi M, Kawai T, Hibi H, Ueda M.
Source
Department of Oral and Maxillofacial Surgery, Nagoya University Graduate, School of Medicine. Electronic address: t-inukai@med.nagoya-u.ac.jp.
Abstract
The effect of conditioned medium from cultured mesenchymal stem cells (MSC-CM) on periodontal regeneration was evaluated. In vitro, MSC-CM stimulated migration and proliferation of dog MSCs (dMSCs) and dog periodontal ligament cells (dPDLCs). Cytokines such as insulin-like growth factor, vascular endothelial growth factor, transforming growth factor-?1, and hepatocyte growth factor were detected in MSC-CM. In vivo, one-wall critical-size, intrabony periodontal defects were surgically created in the mandible of dogs. Dogs with these defects were divided into three groups that received MSC-CM, PBS, or no implants. Absorbable atelo-collagen sponges (TERUPLUG®) were used as a scaffold material. Based on radiographic and histological observation 4 weeks after transplantation, the defect sites in the MSC-CM group displayed significantly greater alveolar bone and cementum regeneration than the other groups. These findings suggest that MSC-CM enhanced periodontal regeneration due to multiple cytokines contained in MSC-CM.
Copyright © 2012. Published by Elsevier Inc.
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